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anti psrc  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti psrc
    Anti Psrc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 2354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+psrc/Phospho-Src+Family+(Tyr416)+Antibody/pmc12975496-86-23-24
    Average 96 stars, based on 2354 article reviews
    anti psrc - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: The fibrocystin C-terminal domain inhibits Src/STAT3 signal induced cystogenesis of kidney epithelia
    Article Snippet: Protein was transferred onto nitrocellulose membrane, Amersham Protran (#10600003; Sigma-Aldrich, Germany) with Towbin buffer (0.18% methanol, 0.01% SDS) and blocked with 5 % BSA in 50 % TBST and 50 % Intercept (TBS) blocking buffer (#927-60001, LI-COR Biosciences, Germany). .. Membranes were incubated overnight at 4° C with anti-pSTAT3 (1:500, #9145, Y705, Cell Signaling Technology), anti-pSrc (1:1000, #6943, Y416, Cell Signaling Technology), anti-STAT3 (1:1000, #9139, Cell Signaling Technology), anti-Src (1: 1000, #2110, Cell Signaling Technology), non-muscle myosin IIa (1:1000, #M806, Sigma), anti-V5 (1:1000, #AB3792, Millipore) and anti-FLAG (2.5:1000, #F7425, Sigma). .. Protein bands were detected using goat anti-rabbit IRDye® 800 CW (1:10,000, #926-32211) or goat anti-rabbit IRDye® 680 RD (1:10,000, #926-68071, LiCOR, USA) via Odyssey Fc Imaging System.

    Gene Expression:

    Article Title: Irisin acts through its integrin receptor in a two-step process involving extracellular Hsp90α
    Article Snippet: The protein concentration of the supernatant was measured by Pierce Detergent Compatible Bradford Assay Reagent (Thermo Scientific 1863028), and 10 μg of total protein from each sample was loaded and resolved by SDS-PAGE. .. Antibodies used for integrin signaling probing: anti-FAK (Cell Signaling 3285), anti-pFAK (Cell Signaling 3283), anti-CREB (Cell Signaling 9104), anti-pCREB (Cell Signaling 9198), anti-Src (Cell Signaling 2108), and anti-pSrc (Cell Signaling 6943). . Gene expression analysis by qRT-PCR Total RNA was isolated from inguinal fat tissues after 45 min of irisin injection using TRIzol reagent (Invitrogen, 15596018) and RNeasy Mini purification kit (Qiagen, 74104) according the manufactures protocol. .. Tissues were homogenized in TRIzol reagent using a bead homogenizer for 20 min at max speed (Qiagen, TissueLyser II).

    Quantitative RT-PCR:

    Article Title: Irisin acts through its integrin receptor in a two-step process involving extracellular Hsp90α
    Article Snippet: The protein concentration of the supernatant was measured by Pierce Detergent Compatible Bradford Assay Reagent (Thermo Scientific 1863028), and 10 μg of total protein from each sample was loaded and resolved by SDS-PAGE. .. Antibodies used for integrin signaling probing: anti-FAK (Cell Signaling 3285), anti-pFAK (Cell Signaling 3283), anti-CREB (Cell Signaling 9104), anti-pCREB (Cell Signaling 9198), anti-Src (Cell Signaling 2108), and anti-pSrc (Cell Signaling 6943). . Gene expression analysis by qRT-PCR Total RNA was isolated from inguinal fat tissues after 45 min of irisin injection using TRIzol reagent (Invitrogen, 15596018) and RNeasy Mini purification kit (Qiagen, 74104) according the manufactures protocol. .. Tissues were homogenized in TRIzol reagent using a bead homogenizer for 20 min at max speed (Qiagen, TissueLyser II).

    Isolation:

    Article Title: Irisin acts through its integrin receptor in a two-step process involving extracellular Hsp90α
    Article Snippet: The protein concentration of the supernatant was measured by Pierce Detergent Compatible Bradford Assay Reagent (Thermo Scientific 1863028), and 10 μg of total protein from each sample was loaded and resolved by SDS-PAGE. .. Antibodies used for integrin signaling probing: anti-FAK (Cell Signaling 3285), anti-pFAK (Cell Signaling 3283), anti-CREB (Cell Signaling 9104), anti-pCREB (Cell Signaling 9198), anti-Src (Cell Signaling 2108), and anti-pSrc (Cell Signaling 6943). . Gene expression analysis by qRT-PCR Total RNA was isolated from inguinal fat tissues after 45 min of irisin injection using TRIzol reagent (Invitrogen, 15596018) and RNeasy Mini purification kit (Qiagen, 74104) according the manufactures protocol. .. Tissues were homogenized in TRIzol reagent using a bead homogenizer for 20 min at max speed (Qiagen, TissueLyser II).

    Injection:

    Article Title: Irisin acts through its integrin receptor in a two-step process involving extracellular Hsp90α
    Article Snippet: The protein concentration of the supernatant was measured by Pierce Detergent Compatible Bradford Assay Reagent (Thermo Scientific 1863028), and 10 μg of total protein from each sample was loaded and resolved by SDS-PAGE. .. Antibodies used for integrin signaling probing: anti-FAK (Cell Signaling 3285), anti-pFAK (Cell Signaling 3283), anti-CREB (Cell Signaling 9104), anti-pCREB (Cell Signaling 9198), anti-Src (Cell Signaling 2108), and anti-pSrc (Cell Signaling 6943). . Gene expression analysis by qRT-PCR Total RNA was isolated from inguinal fat tissues after 45 min of irisin injection using TRIzol reagent (Invitrogen, 15596018) and RNeasy Mini purification kit (Qiagen, 74104) according the manufactures protocol. .. Tissues were homogenized in TRIzol reagent using a bead homogenizer for 20 min at max speed (Qiagen, TissueLyser II).

    Purification:

    Article Title: Irisin acts through its integrin receptor in a two-step process involving extracellular Hsp90α
    Article Snippet: The protein concentration of the supernatant was measured by Pierce Detergent Compatible Bradford Assay Reagent (Thermo Scientific 1863028), and 10 μg of total protein from each sample was loaded and resolved by SDS-PAGE. .. Antibodies used for integrin signaling probing: anti-FAK (Cell Signaling 3285), anti-pFAK (Cell Signaling 3283), anti-CREB (Cell Signaling 9104), anti-pCREB (Cell Signaling 9198), anti-Src (Cell Signaling 2108), and anti-pSrc (Cell Signaling 6943). . Gene expression analysis by qRT-PCR Total RNA was isolated from inguinal fat tissues after 45 min of irisin injection using TRIzol reagent (Invitrogen, 15596018) and RNeasy Mini purification kit (Qiagen, 74104) according the manufactures protocol. .. Tissues were homogenized in TRIzol reagent using a bead homogenizer for 20 min at max speed (Qiagen, TissueLyser II).

    Western Blot:

    Article Title: Liver mechanosignaling as a natural anti-hepatitis B virus mechanism
    Article Snippet: .. The antibodies used for western blot, immunofluorescence, and immunohistochemistry were: Anti-YAP (14074, Cell signaling technology), Anti-pYAP (ab76252, Abcam), Anti-TAZ (83669, Cell signaling technology), Anti-MST1 (3682, Cell signaling technology), Anti-MST2 (3952, Cell signaling technology), Anti-pMST1/MST2 (49332, Cell signaling technology), Anti-LATS1 (3477, Cell signaling technology), Anti-pLATS1 (9157, Cell signaling technology), AntiSrc (2109, Cell signaling technology), Anti-pSrc (6943, Cell signaling technology), Anti-FAK (13009, Cell signaling technology), AntipFAK (3283, Cell signaling technology), Anti-E-Cadherin (3195, Cell signaling technology), Anti-N-Cadherin (13116, Cell signaling technology), Anti-Erk1/2 (9102, Cell signaling technology), Anti-pErk1/2 (9101, Cell signaling technology), Anti-Akt (9272, Cell signaling technology), Anti-pAkt (4060, Cell signaling technology), Anti-HNF4A (K9218, Thermo fisher), Anti-Albumin (PA5-85166, Thermo fisher), Anti-!SMA (19245, Cell signaling technology), Anti-Ki67 (ab16667 Abcam), Anti-CK19 (ab52625, Abcam), Anti-HBcAg (PA367, Abcarta), Anti-HBsAg (PA368, Abcarta), Anti-GAPDH (5174, Cell signaling technology), Anti-rabbit IgG (Alexa Fluor 555 Conjugate) (4413, Cell signaling technology), Anti-mouse IgG (Alexa Fluor 488 Conjugate) (4408, Cell signaling technology) All antibodies were commercially obtained and validated by vendors and multiple published studies, see manufacture's website for references. .. The hepatoma cell lines Huh-7 were obtained from the ATCC.

    Immunofluorescence:

    Article Title: Liver mechanosignaling as a natural anti-hepatitis B virus mechanism
    Article Snippet: .. The antibodies used for western blot, immunofluorescence, and immunohistochemistry were: Anti-YAP (14074, Cell signaling technology), Anti-pYAP (ab76252, Abcam), Anti-TAZ (83669, Cell signaling technology), Anti-MST1 (3682, Cell signaling technology), Anti-MST2 (3952, Cell signaling technology), Anti-pMST1/MST2 (49332, Cell signaling technology), Anti-LATS1 (3477, Cell signaling technology), Anti-pLATS1 (9157, Cell signaling technology), AntiSrc (2109, Cell signaling technology), Anti-pSrc (6943, Cell signaling technology), Anti-FAK (13009, Cell signaling technology), AntipFAK (3283, Cell signaling technology), Anti-E-Cadherin (3195, Cell signaling technology), Anti-N-Cadherin (13116, Cell signaling technology), Anti-Erk1/2 (9102, Cell signaling technology), Anti-pErk1/2 (9101, Cell signaling technology), Anti-Akt (9272, Cell signaling technology), Anti-pAkt (4060, Cell signaling technology), Anti-HNF4A (K9218, Thermo fisher), Anti-Albumin (PA5-85166, Thermo fisher), Anti-!SMA (19245, Cell signaling technology), Anti-Ki67 (ab16667 Abcam), Anti-CK19 (ab52625, Abcam), Anti-HBcAg (PA367, Abcarta), Anti-HBsAg (PA368, Abcarta), Anti-GAPDH (5174, Cell signaling technology), Anti-rabbit IgG (Alexa Fluor 555 Conjugate) (4413, Cell signaling technology), Anti-mouse IgG (Alexa Fluor 488 Conjugate) (4408, Cell signaling technology) All antibodies were commercially obtained and validated by vendors and multiple published studies, see manufacture's website for references. .. The hepatoma cell lines Huh-7 were obtained from the ATCC.

    Immunohistochemistry:

    Article Title: Liver mechanosignaling as a natural anti-hepatitis B virus mechanism
    Article Snippet: .. The antibodies used for western blot, immunofluorescence, and immunohistochemistry were: Anti-YAP (14074, Cell signaling technology), Anti-pYAP (ab76252, Abcam), Anti-TAZ (83669, Cell signaling technology), Anti-MST1 (3682, Cell signaling technology), Anti-MST2 (3952, Cell signaling technology), Anti-pMST1/MST2 (49332, Cell signaling technology), Anti-LATS1 (3477, Cell signaling technology), Anti-pLATS1 (9157, Cell signaling technology), AntiSrc (2109, Cell signaling technology), Anti-pSrc (6943, Cell signaling technology), Anti-FAK (13009, Cell signaling technology), AntipFAK (3283, Cell signaling technology), Anti-E-Cadherin (3195, Cell signaling technology), Anti-N-Cadherin (13116, Cell signaling technology), Anti-Erk1/2 (9102, Cell signaling technology), Anti-pErk1/2 (9101, Cell signaling technology), Anti-Akt (9272, Cell signaling technology), Anti-pAkt (4060, Cell signaling technology), Anti-HNF4A (K9218, Thermo fisher), Anti-Albumin (PA5-85166, Thermo fisher), Anti-!SMA (19245, Cell signaling technology), Anti-Ki67 (ab16667 Abcam), Anti-CK19 (ab52625, Abcam), Anti-HBcAg (PA367, Abcarta), Anti-HBsAg (PA368, Abcarta), Anti-GAPDH (5174, Cell signaling technology), Anti-rabbit IgG (Alexa Fluor 555 Conjugate) (4413, Cell signaling technology), Anti-mouse IgG (Alexa Fluor 488 Conjugate) (4408, Cell signaling technology) All antibodies were commercially obtained and validated by vendors and multiple published studies, see manufacture's website for references. .. The hepatoma cell lines Huh-7 were obtained from the ATCC.

    SDS Page:

    Article Title: Inhibition of glycolysis and Src/Akt signaling reduces Caveolin-1-enhanced metastasis.
    Article Snippet: .. Protein samples were separated by SDS-PAGE (50 μg/lane), transferred to nitrocellulose, blocked for 1 h in blocking buffer (1 % Tween-20 containing 5 % bovine serum albumin) and probed overnight at 4◦C with anti-CAV1 (1:5000, #610060, BD, San Jose, CA, USA), anti-pAkt (1:500, #4060 and #4056, CST, Danvers, USA), anti-Akt (1:500, #9272, CST, Danvers, USA), anti-pSrc (1:1000, #2101, CST, Danvers, USA) antibodies diluted in blocking buffer. .. Anti-β-actin (1:3000, A5060, SigmaAldrich, St. Louis, MO, USA) or anti-HSP90 (1:3000, sc-515081, Santa Cruz Biotechnology, Dallas, Texas, USA) antibodies were used to confirm equal protein loading in the different wells.

    Blocking Assay:

    Article Title: Inhibition of glycolysis and Src/Akt signaling reduces Caveolin-1-enhanced metastasis.
    Article Snippet: .. Protein samples were separated by SDS-PAGE (50 μg/lane), transferred to nitrocellulose, blocked for 1 h in blocking buffer (1 % Tween-20 containing 5 % bovine serum albumin) and probed overnight at 4◦C with anti-CAV1 (1:5000, #610060, BD, San Jose, CA, USA), anti-pAkt (1:500, #4060 and #4056, CST, Danvers, USA), anti-Akt (1:500, #9272, CST, Danvers, USA), anti-pSrc (1:1000, #2101, CST, Danvers, USA) antibodies diluted in blocking buffer. .. Anti-β-actin (1:3000, A5060, SigmaAldrich, St. Louis, MO, USA) or anti-HSP90 (1:3000, sc-515081, Santa Cruz Biotechnology, Dallas, Texas, USA) antibodies were used to confirm equal protein loading in the different wells.

    other:

    Article Title: Irisin acts through its integrin receptor in a two-step process involving extracellular Hsp90α
    Article Snippet: Antibodies used for integrin signaling probing: anti-FAK (Cell Signaling 3285), anti-pFAK (Cell Signaling 3283), anti-CREB (Cell Signaling 9104), anti-pCREB (Cell Signaling 9198), anti-Src (Cell Signaling 2108), and anti-pSrc (Cell Signaling 6943).



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